Hypoglycemic activity of the total of phytoecdysteroids isolated from Silene tomentella

Abstract

Phytoecdysteroids tomentesterone A(1), tomentesterone B(2), viticosterone E(3), 2-deoxy-α-ecdysone(4), polypodine B(5), 20-hydroxyecdysone(6), integristerone A(7), sileneoside D(8), 2,3,20,22-diacetonide 20-hydroxyecdysone(9), and cyasterone(10) were isolated from Silene tomentella. Experiments on intact rats showed their hypoglycemic activity. The experiments showed that the SPE leads to a certain reliable hypoglycemic effect expressed in relation to the initial level in 3 hours after introduction (p<0.05). Blood sugar decreased by 17.6% (baseline - 5.1 ± 0.33, in 3 hours 4.2 ± 0.20 mM/L). In animals with hyperglycemia caused by the administration of glucose and alloxan the SPE also exerted a pronounced sugar-lowering effect. At that, in the group of animals with the blood sugar level of 8.0 mM/L the hypoglycemic activity of the tested sum was less pronounced than in glibenclamide, but in animals with blood sugar levels up to 14 mM/L there were no reliable differences in hypoglycemic activity between both groups. In rats of alloxan group with blood sugar level up to 20 mM/L after the introduction of the SPE there was even a slight advantage over glibenclamide in the hypoglycemic activity in 60 and 120 minutes after introduction, and in 180 minutes this difference generally acquired a reliable character.