Development and Optimization of Antibody and Protein Purification Workflows for Research and Diagnostic Applications

Authors

  • David Aphkhazava PhD, Professor, University of Georgia, Tbilisi, Georgia. Orcid: https://orcid.org/0000- 0001- 6216-64
  • Archil Chirakadze PhD, Georgian Technical University Institute "Techinform", Tbilisi, Georgia, Georgian Technical University Institute of Cybernetics, Tbilisi, Georgia, Ivane Javakhishvili Tbilisi State University Institute of Physics, Tbilisi, Georgia
  • Levan Gulua PhD, Professor, Head of bachelor program of Biomedicine at University of Georgia, Tbilisi, Georgia
  • Mzia Tsiklauri PhD, Affiliated Professor of the Medical Programs of Gr.Robakidze University, Microbiology, Immunology, Virology, Infection Control. Invited Professor of the Medical Programs of Alte University, Tbilisi, Georgia. Invited Professor of the Medical Programs of Caucasus International University, Laboratory Medicine, Tbilisi, Georgia. Member of the Georgian Immunologists Association, Member of the Accreditation Council of the Quality Development, Center of the Ministry of Education of Georgia
  • Manana Makharadze Prof. David Agmashenebeli University of Georgia, Tbilisi, Georgia.
  • Maia Berodze Assistant Professor at Caucasus International University, Tbilis, Georgia
  • Ketevan Chakhnashvili Clinical Director at Pineo Medical Ecosystem. Vice Dean of School of Medicine at Grigol Robakidze University. Tbilisi, Georgia
  • Nodar Sulashvili MD, PhD, Doctor of Pharmaceutical and Pharmacological Sciences In Medicine, Invited Lecturer (Professor) of Scientific Research-Skills Center at Tbilisi State Medical University; Professor of Medical and Clinical Pharmacology of International School of Medicine at Alte University; Professor of Pharmacology of Faculty of Medicine at Georgian National University SEU, Associate Affiliated Professor of Medical Pharmacology of Faculty of Medicine at Sulkhan-Saba Orbeliani University; Associate Professor of Medical Pharmacology at School of Medicine at David Aghmashenebeli University of Georgia; Associate Professor of Biochemistry and Pharmacology Direction of School of Health Sciences at the University of Georgia. Associate Professor of Pharmacology of Faculty of «Research Retrieval and Academic Letters» (April 2-3, 2026). Warsaw, Poland 427 Dentistry and Pharmacy at Tbilisi Humanitarian Teaching University; Tbilisi, Georgia; Orcid: https://orcid.org/0000-0002-9005-8577.
  • Cezar Goletiani Professor at Free University of Tbilisi, Tbilisi, Georgia, Head scientist at Agricultural University of Georgia, Tbilisi, Georgia
  • Nino Nebieridze Associate Professor at Free University of Tbilisi, Tbilisi, Georgia
  • Lolita Shengelia PhD, Invited lecturer of Georgian National University, Tbilisi, Georgia; Invited lecturer of Georgian American University, Tbilisi, Georgia
  • Mariam Iagorashvili Tbilisi State Medical University, Tbilisi, Georgia
  • George Maglakelidze PhD, Professor, University of Georgia, Tbilisi, Georgia
  • Ilia Atanelishvili Medical University of South Carolina, Charleston, SC, USA

Keywords:

Antibody development, protein extraction, protein isolation, protein purification, protein analysis, laboratory techniques, biotechnology research, biomolecular methods, protein workflows, diagnostic applications

Abstract

This work describes laboratory-based research focused on antibody production and the isolation, purification, and characterization of proteins for biomedical applications. The study involved the use of experimental workflows for protein extraction, purification optimization, and assessment of protein quality, purity, and usability in downstream applications. Antibody-focused procedures were integrated into the workflow to support detection-related and analytical research objectives.
The project reflects strong practical experience in working with proteins across multiple experimental stages, from sample preparation to purification and biochemical evaluation. Emphasis was placed on reproducibility, preservation of protein functionality, and optimization of laboratory procedures for efficient handling of protein samples. This work demonstrates applied expertise in antibody-related research, protein isolation, and protein analysis, and highlights technical competencies highly relevant to modern biotechnology and life science laboratories.

Published

2026-04-20

How to Cite

David Aphkhazava, Archil Chirakadze, Levan Gulua, Mzia Tsiklauri, Manana Makharadze, Maia Berodze, Ketevan Chakhnashvili, Nodar Sulashvili, Cezar Goletiani, Nino Nebieridze, Lolita Shengelia, Mariam Iagorashvili, George Maglakelidze, & Ilia Atanelishvili. (2026). Development and Optimization of Antibody and Protein Purification Workflows for Research and Diagnostic Applications. Modern Scientific Technology, (13). Retrieved from https://ojs.publisher.agency/index.php/MSC/article/view/8375

Issue

No. 13 (2026): Modern Scientific Technology

Section

Biological Sciences

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.