Study of the potential for enhancing environmental efficiency in the production of a bioadditive from alfalfa in vitro culture

Abstract

This research aims to explore the induction of alfalfa callus tissues without the use of synthetic growth regulators, thereby aligning with Kazakhstan’s broader environmental policies. The specific objectives of this study include: (1) optimizing in vitro culture conditions using natural biostimulants, (2) comparing the effectiveness of various natural alternatives in promoting callus formation, and (3) evaluating the potential applications of the produced biomass in the food and feed industries.

To achieve these objectives, a combination of in vitro culture techniques and analytical methods will be employed. By optimizing culture conditions and utilizing natural biostimulants, this research contributes to the scientific community’s understanding of sustainable biotechnological practices while supporting Kazakhstan's goals of fostering ecological sustainability and innovation in agricultural production.