Development of technology for obtaining a probiotic strain of Lactobacillus bulgaricus C-2 promising for the production of starter cultures and biologically active additives
Keywords:
Lactobacillus bulgaricus, nutrient medium, cultivation, concentration, sublimation, cryoprotectorsAbstract
Lactic acid probiotic bacteria can be widely used for the prevention and treatment of patients with various diseases, such as acute and chronic diseases of the gastrointestinal tract, respiratory tract, to restore intestinal microbiocenosis, in combination with antibiotic therapy. The form of reception of these microorganisms is diverse: fermented milk products, medicines, biologically active additives.
In the production of lactic acid starter cultures, including the probiotic strain Lactobacillus bulgaricus, problems arise in the selection of nutrient media and conditions for deep cultivation, the selection of methods and conditions of concentration, in the selection of cryoprotectors for freeze drying.
This article presents all the data for the production of Lactobacillus bulgaricus C-2 strain in powdered form with the maximum yield of microorganism cells, promising for introduction into the composition of starter cultures for the dairy industry and biologically active additives with probiotic effect. The strain Lactobacillus bulgaricus C-2 was isolated from koumiss and is stored in the collection of the branch of the RSE at the PHW of the Ministry of Health of the Republic of Kazakhstan "National Center of Biotechnology" in Stepnogorsk. The production nutrient medium, conditions for deep cultivation, methods and conditions of concentration were selected, optimal cryoprotectors for freeze drying were selected